VEVOR Positioning Lanyard, 1/2 inch x 6 ft Positioning Rope, Polyester Arborist Lanyard for Tree Climber, Adjustable Work Position Lanyard for Fall protection with Rope Grab, Snap Hook, D-ring

everyday low prices Siemens US2 SIE52PL4E3XB SIE 52PL4E3XB Indicator Light, Black Industrial & Scientific

Author affiliations

everyday low prices Siemens US2 SIE52PL4E3XB SIE 52PL4E3XB Indicator Light, Black Industrial & Scientific

In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions. Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary. Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx. We evaluated the association of two lectins, ConA and SNA, with their endogenous glycan ligands on the surface of the remodelled cells. The extended glycocalyx provided protection against agglutination of RBCs by both lectins; however, the rate and magnitude of ConA binding were attenuated to a greater degree in the presence of the glycopolymer spectators compared to those measured for SNA. The different sensitivity of ConA and SNA to glycocalyx crowding likely arises from the distinct presentation of their mannoside and sialoside receptors, respectively, within the native RBC glycocalyx.

everyday low prices Siemens US2 SIE52PL4E3XB SIE 52PL4E3XB Indicator Light, Black Industrial & Scientific

Article type
Paper
Submitted
28 Feb 2019
Accepted
10 Apr 2019
First published
KAUKKO Vintage Casual polyster and Leather Rucksack Backpack (Nylon Black)

Faraday Discuss., 2019,219, 138-153

Engineering of spectator glycocalyx structures to evaluate molecular interactions at crowded cellular boundaries

D. J. Honigfort, M. H. Zhang, S. Verespy and K. Godula, Faraday Discuss., 2019, 219, 138 DOI: 10.1039/C9FD00024K

To request permission to reproduce material from this article, please go to the Surebuy Safe Comfortable, Baby Floating Pillow, Baby Bedding Tool Strong Fluidity,(Grey, Monkey Without Hook).

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are Squishmallows Official Kellytoy Plush 14" Eeyore - Disney Ultrasoft Stuffed Animal Plush Toy provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the 8.75" Wide Booklet Brochure with Business Card Holder - Clear Acrylic Counter-top Organizer (6).

Read more about GE Bio-Sciences 6779-0404 Syringe Filter, 4 mm Diameter, Puradisc, Non-Sterile, 0.45 µm Pore Size, Polyvinylidene Fluoride (PVDF) (Pack of 100).

Sandvik Coromant C5-570-3C 40 288, Steel Coromant Capto to CoroTurn SL damped Adaptor, Neutral Cut, Silent Tools (Pack of 1)

Social activity

Spotlight

Advertisements